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Rev. cuba. med. trop ; 62(1): 56-65, ene.-abr. 2010.
Article in Spanish | LILACS | ID: lil-584933

ABSTRACT

INTRODUCCIÓN: las diarreas agudas presentan elevada morbilidad y mortalidad en los países en vías de desarrollo y Escherichia coli diarreogénicos aparecen como agentes etiológicos frecuentes en todos ellos. OBJETIVO: estudiar la presencia de los diferentes tipos de Escherichia coli diarreogénicos en niños que concurrieron a Centros de Salud de barrios humildes de la ciudad de Corrientes, Argentina. MÉTODOS: se cultivaron heces de 120 pacientes con diarrea aguda y en aquellas muestras negativas para Salmonella y Shigella se seleccionaron aislamientos de E. coli, cuyos factores de patogenicidad fueron estudiados mediante reacción en cadena de la polimerasa múltiple. RESULTADOS: los 112 pacientes estudiados habitaban viviendas urbanas, con suministro de agua potable y baño instalado. En 41 muestras (37 por ciento) se detectó algún tipo patogénico de Escherichia coli diarreogénico sin predominio marcado de ninguno de ellos. No se hallaron diferencias significativas entre la presencia de estos y el sexo o el grupo etario. CONCLUSIONES: Escherichia coli diarreogénico aparece como un agente etiológico habitual en niños y el reconocimiento de cada tipo implicado permitirá implementar medidas preventivas acordes con la epidemiología de cada uno de ellos.


INTRODUCTION: acute diarrheas bring high morbidity and mortality in developing countries and diarrheagenic Escherichia coli (DEC) appears as frequent etiological agent in all these places. OBJECTIVE: to detect the presence of pathogenic types of DEC in children attending the Health Centers in poor neighborhoods in the city of Corrientes, Argentina. METHODS: Feces from one hundred and twenty patients were cultured; E. coli isolates were selected in those cases negative for Salmonella or Shigella, and they were studied by multiplex PCR in order to characterize the pathogenic types of DEC. RESULTS: one hundred and twelve patients were included. All of them had urban housing with drinking water supply and toilet. In 41 samples (37 percent) some DEC type was detected but none of them (STEC 7, EPEC 7, EIEC 7, EAEC 10, and ETEC 10) was predominant. There were not significant differences between the DEC pathogenic types and the sex or the age group of patients. CONCLUSIONS: DEC is frequent etiologic agent in children and the recognition of each pathogenic type will allow implementing prophylactic measures according with its epidemiology.


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Infant , Male , Diarrhea/microbiology , Escherichia coli Infections/complications , Escherichia coli Infections/microbiology , Argentina , Poverty , Residence Characteristics
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